CoolLED pE-300^ultra

The system comprises a pE-300^ultra Light Source, Control Pod, set of three Excitation Filter Holders and Power Supply. It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

Direct-fit for connecting to a microscope – by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide. An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

For confocal microscopes, the liquid light guide variant offers the ideal replacement to a metal halide light source, minimising photobleaching and phototoxicity when viewing samples before scanning.

The pE-300^ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. When paired with today’s high performance multi-band filter sets, this facilitates imaging traditionally achieved using a white light source and a filter wheel, with all the benefits of LEDs – and most excitingly at speeds not previously so affordable. For live cell imaging applications, events can now be captured that might previously have been missed.

A benefit of the pE-300^ultra is that stains can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether.

Fluorophores can be viewed either individually or in combination, without filter cube changes. This makes the pE-300^ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

It is now possible to run through a sequence of excitation channels using a camera or other external hardware which has only a single TTL-out, thanks to the Sequence Runner mode. Complex illumination protocols can now be run at high speeds and without the need specialised and costly hardware. The order of each fluorophore to be captured is defined via the simple Control Pod, with the pE-300^ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the Light Source.

Manual: Manual control for instant on/off and intensity control in 1% steps from 0 – 100% Individual bands can be selected/deselected and controlled independently as desired

Remote: Via global and individual channel TTL for on/off control of selected channels using a BNC connection on the light source. Triggering speed <20us at full power

Connectivity: Remote via USB (B type) for imaging software control

Sequence Runner: Single TTL input to step through user defined sequence. Triggering speed <20us at full power

When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the pE-300ultra at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts. “Green Grants” are even sometimes available from establishments, providing financial support owing to the reduction in institutional energy usage.

Download further information on the CoolLED pE-300^ultra.