Scientifica HoloStim-3D
Interrogate larger neural networks at higher resolution than ever before, with the world's highest performing multiphoton all-optical solution.
The HoloStim-3D seamlessly integrates with the HyperScope and VistaScope award-winning multiphoton imaging systems, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.
Product benefits
Mouse layer ii/iii neurons expressing GCaMP6s (calcium indicator) and C1V1 (red-shifted channelrhodopsin) are simultaneously stimulated using the HoloStim-3D, while their calcium responses are imaged using resonance two-photon imaging on the HyperScope. In the total 100 cells were stimulated at 6 nJ per cell, using a spiral pattern. Data acquired by Kelly Sakaki (Scientifica), Robert Lees and Adam Packer (both University of Oxford).
Investigate neuronal circuits with 3D precision
The HoloStim-3D enables you to perform more complex photostimulation experiments, with higher throughput than those that are possible with random-access two-photon or widefield photostimulation.
Discover the roles of neuronal subtypes and brain circuits by simultaneously targeting multiple cells with cellular resolution.
Observe and record comprehensive network activity by combining precise cell stimulation and imaging with the HoloStim-3D.
By selectively photostimulating only the target cells and rapidly switching between stimulation patterns, you can better understand neuronal interactions in networks.
Software
Adopting SLM technology is made easier with full integration of globally-recognised ScanImage software, saving you time so you can start experimenting faster.
Scientifica services for this product
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